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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells
doi: 10.2147/IJN.S132762
Figure Lengend Snippet: Western blot and NTA validation of exosomal samples. Notes: Western blots of ( A ) breast milk exosomes (60 μg/lane) [(A1) clathrin and (A2) CD9] and ( B , C ) plasma exosomes (25 μg/lane) [(B1) CD9, (B2) CD63], ( C ) TIM-4. Arrows indicate proteins of interest. ( D , E ) NTA-generated size and concentration plots for ( D ) human plasma- and ( E ) human breast milk-derived exosomes. Abbreviations: NTA, nanoparticle tracking analysis; TIM, T cell immunoglobulin and mucin.
Article Snippet: A protocol similar to HIV-1 infection was performed but with addition of 0.2 μg/well anti-mouse TIM-4 or
Techniques: Western Blot, Generated, Concentration Assay, Derivative Assay
Journal: International Journal of Nanomedicine
Article Title: Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells
doi: 10.2147/IJN.S132762
Figure Lengend Snippet: NSC- and A549-derived exosomes significantly enhance HIV-1 entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) NSC-derived exosomes (0.1 μg) or ( C ) A549-dervied exosomes (0.1 μg). ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) NSC-derived exosomes (0.1 μg) or ( D ) A549-derived exosomes (0.1 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA * P <0.05, *** P <0.001, **** P <0.0001. YU-2, (NL-LucR.T2A-YU2.ecto) engineered to express Renilla luciferase (LucR) and ENV from the YU-2 virus strain. Abbreviations: NSC, neural stem cell; TIM, T cell immunoglobulin and mucin; ANOVA, analysis of variance; RLU, relative luminescence unit, exo, exosomes.
Article Snippet: A protocol similar to HIV-1 infection was performed but with addition of 0.2 μg/well anti-mouse TIM-4 or
Techniques: Derivative Assay, Expressing, Luciferase, Activity Assay, Infection
Journal: International Journal of Nanomedicine
Article Title: Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells
doi: 10.2147/IJN.S132762
Figure Lengend Snippet: Breast milk- and plasma-derived exosomes enhance HIV-1 entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) breast milk-derived exosomes (0.035 μg) or ( C ) plasma-derived exosomes (0.05 μg). Virus entry was evaluated in the presence of exosomes and anti-TIM-4 antibodies. ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) breast milk-derived exosomes (0.035 μg) or ( D ) plasma-derived exosomes (0.05 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA. ** P <0.01, **** P <0.0001. YU-2, (NL-LucR.T2A-YU2. ecto) engineered to express Renilla luciferase (LucR) and ENV from the YU-2 virus strain. Abbreviations: TIM, T cell immunoglobulin and mucin; ANOVA, analysis of variance; RLU, relative luminescence unit; BM, breast milk; exo, exosomes.
Article Snippet: A protocol similar to HIV-1 infection was performed but with addition of 0.2 μg/well anti-mouse TIM-4 or
Techniques: Derivative Assay, Expressing, Luciferase, Activity Assay, Infection